Tissue Culture Tasks

Tayo

Water Bath and Sonicator

Check weekly for sufficient water levels and for contamination. Remove all water, clean with IPA and replace water along with antibiotics at least once/month.

Person needed

CO2 Tanks

Check the CO2 tanks weekly on Friday. Order new tanks and change over when necessary.

Person needed

Incubator

Check weekly for unlabeled cultures, old cultures (>2 weeks) and cultures with visible contamination. Also change water in water pans (with DI water) and make sure incubator is not alarming. Move everything to other incubator, wipe down with IPA and change water pans. Wait an hour for vapor to dissipate before moving everything back. Change filter when necessary.

Person needed

Solutions

Check weekly and make 70% Ethanol, 70% IPA, 10% Bleach and 7x cleaning solution and fill all squirt/spray bottles when necessary.

Person needed

Frozen Aliquots

Check weekly, order and aliquot HS, FBS and Pen/Strep when necessary. Make up plating media for initial dilution of neonatal rat cardiomyocyte isolation.

Kevin Chung

Waste disposal

Check boxes and sharps container weekly. Close up the sharps box and the Pasteur pipette jug and replace when necessary.

Dan

Sterilization

Run all items in the sterilizing bin through the sterilizer weekly. Ensure neonate cardiomyocyte isolation stuff if sterilized by day of isolation

Person needed

Supplies

Check weekly on the level of 1ml, 2ml, 5ml, 10ml and 25ml pipettes, 15ml and 50ml centrifuge tubes, 20ul, 200ul and 1ml pipette tips and 6-well plates. Make sure drawers and shelves are full in the TC room and make sure more get ordered if needed.

Atsu

Glassware

Weekly put away drying glassware and put down new bench paper in the drying area. Generally clean sink area.

Person needed

Refrigerator

Weekly check through the refrigerator for old or unlabeled items. Everything should be labeled with a name and date at the minimum.

Cell Culture Schedule

June 24

20 rats ordered

  • Atsu -- 20 million cells
  • Amy -- 3 million cells

July 3

  • Karen -- 15 million cells

July 10

  • Karen -- 20 million cells

July 17

  • Karen -- 20 million cells
  • Jennifer -- 70 million cells

July 24

  • Joyce-- 10 million cells
  • Karen -- 40 million cells

July 29

  • Jen -- 10 million cells
  • Joyce -- 10 million cells
  • Jessica -- 50 million cells

July 30

  • Karen -- 40 million cells

August 4

  • Jessica -- 40 million cells

August 5

  • Jen S. -- 20 million cells

August 6

  • Karen -- 40 million cells

August 12

  • Joyce -- 30 million cells

August 12

  • Jen/Rob -- 20 million cells

August 11

  • Jessica -- 40 million cells

August 14

  • Karen -- 20 million cells

August 19

  • Joyce -- 15 million cells
  • Jen/Rob -- 15 million cells

August 26

  • Joyce/Dr. Yang -- 30 million cells
  • Jessica -- 40 million cells

September 2

  • Joyce -- 15 million cells

September 9

  • Dr.Yang -- 15 million cells

September 16

  • Jessica -- 40 million cells
  • Dr.Yang -- 15 million cells

September 23

  • Atsu -- 30 million cells
  • Dr.Yang --21 million cells
  • Joyce -- 10 million cells

October 3

  • Atsu -- 30 million cells

October 10

  • Barbara -- 10 million cells
  • Atsu -- 30 million cells

October 17

  • Atsu -- 40 million cells
  • joyce -- 10 million cells

October 24

  • Barbara -- 10 million cells

October 31

  • Joyce -- 15 million cells

November 14

  • Barbara -- 20 million cells

December 5

  • Barbara -- 30 million cells

December 12

  • Barbara -- 30 million cells

January 23

  • Barbara -- 42 million cells

February 6

  • Barbara -- 28 million cells
  • Atsu -- any extra cells

February 13

  • Atsu -- 40 million cells

February 27

  • Barbara -- 21 million cells

March 6

  • Joyce -- 15 million cells

March 13

  • Barbara -- 12 million cells

March 20

  • Barbara -- 12 million cells

March 27

  • Barbara -- 10 million cells

April 30

  • Barbara -- 10 million cells

May 5

  • Joyce -- 10 million cells